A technology of obtaining microbial protein based on yeast fungi Saccharomyces cerevisiae has been developed. This technology includes preparing of working solution in a form of 10% yeast suspension, activation of nucleases with sodium chloride (0.1 H) under the condition of an acidity of at least 9.0 pH and a temperature of 40 оС for 60 minutes; centrifugation of biomass; separation of culture liquid; introduction of enzymes; drying and grinding. The process of enzymatic hydrolysis of pressed yeast with proteolytic enzyme preparations Protosubtilin, Papain and Bromelain in various dosages (1–5%) to the total mass of raw materials and water has been investigated. The conditions for the fermentation process are as follows: Protosubtilin (6.5 pH, 40 оС for 12 hours), Papain (8.75 pH, 40 оС for 12 hours), Bromelain (5.5 pH, 40 оС for 12 hours). As a result of enzymatic hydrolysis, a high protein yield of 45.6% is observed at 4% Bromelain hydrolysis. When Bromelain is applied in a quantity of 5% the content of free amino acids of proline, cystine and methionine in the resulting protein microbial preparation increases in comparison with the enzyme Protosubtilin. When Papain is applied in a quantity of 4%, the content of aspartic acid, lysine, histidine, and methionine increases compared to Protosubtilin and Bromelain. Consequently, each of the studied enzyme preparations, despite its specificity, has different fermentation ability.

Neustroev Anton P., Tikhonov Sergey L., Tikhonova Natalya V.

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