Studies have been conducted to predict the antioxidant activity of a peptide isolated from pepsin hydrolysate of cow colostrum and its effectiveness has been confirmed in vitro.The peptide was examined on a MALDI-TOPH mass spectrometer, decryption was carried out using the Mascot database, the Peptide Fingerprint option (Matrix Science, USA) using the Protein NCBI database. The antioxidant activity of the peptide was determined by three methods: ABTS, DPPH and FRAP. Analyzing the physico-chemical characteristics of the peptide (composition and quantity of amino acids, hydrophobicity, charge, molecular weight) it is assumed that the isolated peptide is similar to the peptide «Dual specificity protein phosphatase, Bos Taurus», which activates the builtin immunity and has antioxidant activity. The antioxidant activity of the peptide was confirmed by ABTS, DPPH and FRAP methods; and the mechanism of its action based on the suppression of active forms of acid by single electron transfer reactions was suggested. The conducted studies using proteomics methods and a database for predicting the antioxidant activity of a peptide can reduce the time for detecting new properties of bioactive peptides, in particular, antioxidant ones.

Tikhonov Sergey L., Tikhonova Natalya V., Ozhgikhina Anna S., Pestova Inga G.

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