Soybean fungi: A review of open genomic collections and future prospects for molecular diagnostics
Volume 19 • Issue 3
Timkin Pavel D., Penzin Andrey A.
DOI: https://doi.org/10.22450/1999-6837-2025-19-3-28-48
Published on: 15.09.2025
Phytopathogenic fungi pose a serious threat to soybean crops, significantly reducing their yield and quality. This article provides a detailed review of the current status of genomic data for the major soybean phytopathogens: Cercospora sojina, Cercospora kikuchii, Fusarium solani and Septoria glycines. The genomic assemblies available in open sources and their possible applicability for the development of molecular genetic diagnostic test systems in the Amur region of Russia are analyzed. Special attention is paid to the comparative advantages and limitations of different molecular diagnostic methods, such as classical and quantitative PCR, isothermal amplification methods (RPA, LAMP, NASBA, RCA) and combined approaches (CRISPR/Cas systems). PCR technology provides rapid and accurate results but requires laboratory conditions, while isothermal methods offer the possibility of field diagnostics but require more complex primer design. The prospect of using isothermal methods (LAMP, RPA), as well as CRISPR/Cas, is explored due to their high specificity and sensitivity. The described methods provide an opportunity to significantly improve the accuracy and speed of diagnostics, which is critical for timely adoption of soybean protection measures. Key directions for further research are identified, including the need for full genomic sequencing of local strains and the development of specific molecular markers to improve the efficiency of phytopathogen diagnostics.
Timkin P. D., Penzin A. A. Soybean fungi: A review of open genomic collections and future prospects for molecular diagnostics. Dal'nevostochnyi agrarnyi vestnik. 2025;19;3:28–48. (in Russ.). https://doi.org/10.22450/1999-6837-2024-19-3-28-48.
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