The problem of metabolic disorders in the human body, causing obesity and diabetes mellitus, is relevant. An alternative way to prevent and treat these diseases is the use of combined synergistic biologically active agents. Sea buckthorn oil is a natural source of essential and unsaturated fatty acids and other bioactive compounds. Sea buckthorn seeds are characterized by a unique combination of omega-3, 6, 7, 9 fatty acids with anti-toxic and cardio-protective properties. The soft part of the berry and the peel of sea buckthorn is one of the few vegetable sources with a high content of omega-7 monounsaturated fatty acid (43 %), which regulates lipid-carbohydrate metabolism in the body. It is known that probiotic microorganisms play an important role in the prevention and correction of lipid metabolism. In general, studies on the effect of polyunsaturated fatty acids of sea buckthorn oil on probiotic microorganisms are limited. Therefore, sea buckthorn oil and pure cultures of bifidobacteria Bifidobacterium longum DK-100 were chosen to create a multifunctional dietary supplement. In this paper, the effect of polyunsaturated fatty acids of sea buckthorn oil on the biochemical activity of bifidobacteria was studied. The most favorable concentration of sea buckthorn oil of 3 % was found to increase the number of bifidobacteria cells during cultivation. It has been proved that the storability of the bacterial concentrate is prolonged in the presence of sea buckthorn oil. The viable status of bifidobacteria cells is hundreds of billions cells in 1 cm3. Probably, polyunsaturated fatty acids of sea buckthorn oil are included in bacterial cell metabolism as a component of membrane phospholipids and storage substances.

Khazagaeva Sofia N., Zambalova Natalia A., Kachanina Lyudmila M., Khamagaeva Irina S.

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