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Far eastern agricultural journal
ISSN 1999-6837 (Print) ISSN 2077-9089 (Online)
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Far eastern agricultural journal

Molecular screening of resistance gene marker to the anthracnose black rot (the Rca2 gene) of strawberry genetic collection

Volume 16 • Issue 3

Lyzhin Alexander S., Lukyanchuk Irina V.

DOI: https://doi.org/10.22450/199996837_2022_3_12

Published on: 14.09.2022

Abstract

Anthracnose (C. acutatum) is a dangerous strawberry disease. The creation of varieties resistant to anthracnose is an important direction of strawberry breeding. Using the diagnostic DNA marker STS-Rca2_240, the Rca2 gene, which controls strawberry resistance to C. acutatum isolates of pathogenicity group 2, was identified in four strawberry varieties, which was 16.7 % of the total number of analyzed genotypes. Among Russian strawberry genotypes, the Rca2 gene was detected in the variety Borovitskaya. Among foreign strawberry genotypes, the Rca2 gene was detected in the varieties Laetitia, Portola and Selva. The remaining analyzed strawberry genotypes are characterized by the homozygous state of the rca2 allele and, therefore, susceptibility to anthracnose. In the Borovitskaya variety, the Rca2 resistance allele is present in the heterozygous form. In the Laetitia, Portola and Selva varieties, the Rca2 resistance allele is present in homozygous or heterozygous form. These strawberry varieties are recommended as promising genetic sources of the Rca2 gene in breeding for resistance to C. acutatum pathogenicity group 2.

For citation

Lyzhin A. S., Lukyanchuk I. V. Molekulyarnyj skrining markera gena ustojchivosti k antraknoznoj chyornoj gnili (gen Rca2) geneticheskoj kollekcii zemlyaniki [Molecular screening of resistance gene marker to the anthracnose black rot (the Rca2 gene) of strawberry genetic collection]. Dal’nevostochnyj agrarnyj vestnik. – Far Eastern Agrarian Bulletin. 2022; 16;3: 12–19. (in Russ.). doi: 10.22450/199996837_2022_3_12.

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