A bioinformatics approach to characterize biological functions of protein
Volume 20 • Issue 1
Tikhonov Sergey L., Kovalnogova Sonya A., Tikhonova Natalya V., Timofeeva Maria S.
DOI: https://doi.org/10.22450/1999-6837-2026-20-1-97-104
Published on: 02.03.2026
The biological value, functional and technological properties of rapeseed, chickpea, and hemp proteins are of strategic importance as promising plant-based protein raw materials for food production. Therefore, the aim of this research is to study the characteristics and properties of the protein sequences of chickpea, rapeseed, and hemp using the UniProt database. The research objects were the plant proteins of rapeseed, chickpea, and hemp: napin 1.7S; napin-B1.7S; 26S proteasome, non-ATPase regulatory subunit 1; non-specific lipid transfer protein; bifunctional inhibitor/plant lipid transfer protein; protein containing a helical seed storage domain. After searching for protein sequences using the UnitProt ID database, we determined the amino acid sequences, biological functions, molecular weight, and number of isoforms of the proteins under study. A bioinformatics search for rapeseed identified 549 proteins, of which two key storage proteins (Napin 1.7S and Napin-B1.7S) were analyzed in detail. Chickpea seeds were found to contain 102 proteins, two of which were examined in the study (26S proteasome regulatory subunit and a non-specific lipid transfer protein). Hemp was found to contain 194 proteins, of which two storage proteins with similar sequences, classified as bifunctional inhibitors/lipid transfers, were selected for study.
Tikhonov S. L., Kovalnogova S. A., Tikhonova N. V., Timofeeva M. S. A bioinformatics approach to characterize biological functions of protein. Dal'nevostochnyi agrarnyi vestnik. 2026;20;1:97–104 (in Russ.). https://doi.org/10.22450/1999-6837-2026-20-1-97-104.
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