The article presents the results of the experiments on common buckwheat carried out using selective medium with high content of cooper sulfate in the Laboratory of Agricultural Biotechnology at Federal Scientific Center of Agrobiotechnology of the Far East named after A. K. Chaika. The effect of copper ions on the regeneration of microplants of the variety Izumrud and the hybrid Izumrud×Inzerskaya were studied in the course of the laboratory tests. Under in vitro conditions different reactions of genotypes to ionic stress were identified. It was found that the plants of the variety Izumrud had stronger resistance to high concentrations of copper salt than hybrid plants did. The microshoots of the variety Izumrud maintained viability after exposure to the media with the cooper sulfate concentrations of 161 and 184 mg/l, while hybrid microshoots did not. The obtained regenerated plants were tolerant to cooper and had a higher capacity for rutin biosynthesis. The plants of the variety Izumrud accumulated a maximum rutin content (2.77 %) after cultivation on the selective medium with the cooper sulfate concentration of 184 mg/l, while plants of hybrid Izumrud×Inzerkaya accumulated 2,73 % of rutin after cultivation on the selective medium with the copper sulfate concentration of 69 mg/l. The studies have shown that the addition of high concentrations of heavy metals to the nutrient medium as a mutagenic component increases the variability and the possibility of obtaining genetic variations that are valuable for selection of traits. Active synthesis of flavonoids in plants is linked to the strengthening of resistance to toxic agents and other stress factors of environment. Breeding of buckwheat plants with high rutin content at the stage of in vitro can improve effectiveness of work on obtaining source material capable of adaptation to stress conditions.

Barsukova Elena N., Klykov Aleksey G.

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