The peculiarity of the immune system of the gastrointestinal tract is that it is in contact with a large amount of microbial and allergic material. The lymphoid tissue of the in testine serves as the first barrier to the penetration of dangerous agents into the body. The main components of the intestinal immune system are Peyer's plaques, which are aggregated lymphoid follicles covered with epithelium. In an experiment conducted on rabbits of the California breed (60 males aged 45 days), the effect of bifidogenic feed additives Lactulose Concentrate "Lactusan" and "Lactulose with glycine" on the number and size of Peyer plaques in the small intestine of rabbits was studied. The control group and two experimental groups (of 20 heads each) were formed by the method of analog pairs. The dry type of feeding was used using full-grain granular compound feed KK-92. The basic value of the norm for the introduction of lactulose into the rabbit diet for a series of planned experiments was 0.06 g/kg of live weight of a rabbit per day. To study Peyer's plaques, the small intestine was separated, cut along the bryzzheika and washed. The number of clearly formed plaques visible to the naked eye was calculated and their measurements were made with a caliper. Then their areas were calculated. The data obtained confirm the conclusions of some researchers that Peyer's plaques are laid only in the early embryonic period, then the appearance of new plaques does not occur. The use of feed bifidogenic additives Lactulose concentrate "Lactusan" and "Lactulose with glycine" in the diet does not increase the number of Peyer plaques in the small intestine of young rabbits of the California breed, but increases their size and increases the variability of the values of the area of Peyer plaques.

Vinogradova Evgeniya V., Chugreev Mihail K., Kul'makova Nataliya I., Borisova Maria M.

1. Batuev K. M. K voprosu vozrastnoj morfologii Pejerovyh blyashek tonkoj kishki cheloveka [On the question of the age morphology of Peyer's plaques of the human small intestine]. Trudy Permskogo medicinskogo instituta. – Proceedings of the Perm Medical Institute, 1968; 3 (71):30–38 (in Russ.).
2. Panfilov A. B. Osobennosti morfologii i kletochnogo sostava kishechno-associirovannoj limfoidnoj tkani u krasnoj kamchatskoj lisy [Features of morphology and cellular composition of intestinal-associated lymphoid tissue in the red Kamchatka fox]. Rossijskie morfologicheskie vedomosti. – Russian Morphological bulletin, 2000; 1–2: 59–69 (in Russ.).
3. Panfilov A. B. Sintopiya limfoidnoj tkani kishechnika u rysi [Syntopia of intestinal lymphoid tissue in lynx]. Morfologicheskie vedomosti. – Morphological bulletin, 2004; 1–2: 78 (in Russ.).
4. Panfilov A. B., Byakov I. A. Sintopiya limfoidnoj tkani pishchevaritel'nogo kanala u domashnej koshki [Syntopia of the lymphoid tissue of the digestive canal in a domestic cat]. Morfologicheskie vedomosti. – Morphological bulletin, 2004; 1–2: 25–27 (in Russ.).
5. Perfilova E. A. Vliyanie immunomodulyatorov na morfogenez limfoidnoj tkani kishechnika u laboratornyh zhivotnyh [Effect of immunomodulators on morphogenesis of intestinal lymphoid tissue in laboratory animals]. Extended abstract of candidate’s thesis. Saransk, 2008, 18 p. (in Russ.).
6. Petrov R. V. Immunologiya [Immunology], Moskva, Medicina, 1987, 416 p. (in Russ.).
7. Ponomareva V. D. Nekotorye vozrastnye izmeneniya stroeniya tonkoj kishki cheloveka [Some age-related changes in the structure of the human small intestine]. Problemy morfologii. – Problems of morphology, 1958; 32: 66–76 (in Russ.).
8. Rekomenduemye urovni potrebleniya pishchevyh i biologicheski aktivnyh veshchestv [Recommended levels of consumption of food and biologically active substances], Moskva, Federal'nyj centr Gosudarstvennogo sanitarno-epidemiologicheskogo nadzora Ministerstva zdravoohraneniya Rossijskoj Federacii, 2004, 14 p.
9. Sapin M. R. Immunnye struktury pishchevaritel'noj sistemy [Immune structures of the digestive system]. Moskva, Medicina, 1987, 224 p. (in Russ.).
10. Voda pit'evaya [Drinking water]. (2004). TU 2004 docs.cntd.ru Retrieved from https://docs.cntd.ru/document/471854910 (Accessed 10 August 2021) (in Russ.).
11. Koncentrat laktulozy «Laktusan» [Lactulose Concentrate «Laktusan»]. (2009). TU 2009 docs.cntd.ru Retrieved from https://docs.cntd.ru/document/415930995 (Accessed 12 August 2021) (in Russ.).
12. Kormovaya dobavka «Laktuloza s glicinom» [Feed additive «Laktuloza s glicinom»]. (2021). TU 2021 docs.cntd.ru Retrieved from https://docs.cntd.ru/document/415930979 (Accessed 14 August 2021) (in Russ.).
13. Haitov P. M., Pinegin B. V. Immunnaya sistema i zabolevaniya zheludochno-kishechnogo trakta [The immune system and diseases of the gastrointestinal tract]. Vestnik Rossijskoj akademii medicinskih nauk. – Bulletin of the Russian Academy of Medical Sciences, 1997; 11: 13–17 (in Russ.).
14. Hilkova I. N. Razvitie obobshchennyh limfaticheskih follikulov ploda cheloveka [Development of generalized human fetal lymphatic follicles]. Problemy funkcional'noj anatomii. – Problems of functional anatomy, 1964; 3: 25–39 (in Russ.).
15. Hlystova Z. S. Stanovlenie sistemy immunogeneza ploda cheloveka [Formation of the human fetal immunogenesis system]. Moskva, Medicina, 1987, 254 p. (in Russ.).
16. Cikunov A. E. Sbornik formul po matematike [Collection of formulas in mathematics]. Sankt-Peterburg, Piter, 2001, 160 p. (in Russ.).
17. Brayden D. J., Baird A. W. A distinctive electrophysiological 112 signature from the Peyer’s patches of rabbit intestine. British Journal of Pharmacology, 1994; 113: 593–599.
18. Jung C., Hugot J.-P., Barreau F. Peyer’s Patches: The Immune Sensors of the Intestine. International Journal of Inflammation, 2010; 12: 1–12.
19. MacDonald T. S., Spenser J. Ontogeny of the mucosal immune response. Springer-Seminars of Immunopathology, 1990; 2–3 (12): 129–137.
20. Mowat A. M. Anatomical basis of tolerance and immunity to intestinal antigens. Nature Reviews Immunology, 2003; 3: 331–341.
21. Sainte-Marie G., Peng F. S., Pelletier M. Development of the lymph nodes in the very young, and their evolution in the mature, nude rat. Developmental and Comparative Immunology, 1984; 8: 695–710.